BioScience Dictionary

 
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Found Southern blot 1 time.

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1. Southern blot (Southern hybridization, Southern blotting)
A technique used for searching for a specific DNA fragment. The process is as follows: * Separate DNA fragments by gel electrophoresis * change pH of gel to basic , thus allowing disruption of H-bonds * blot gel with nitrocellulose paper * heat paper so as to fix DNA fragments * probe with labeled messenger RNA or cDNA * wash * complementary mRNA/cDNA fragments will have hybridized.