BioScience Dictionary

 
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Ot.

Found Cloning vector 18 times.

Displaying results 1 to 10.

1. Bacteriophage lambda
A bacteriophage (a virus which infects bacteria ) that infects E. coli . It has a complex set of regulatory mechanisms to determine whether it will quietly insert its DNA into the bacterial genome to become dormant and to be reproduced whenever the bacterium reproduces (to lysogenize ), or whether it will hijack the bacterium's cellular machinery to reproduce itself and prepare to infect more bacteria, causing the bacterium to self-destruct shortly after infection (to lyse ). Lambda is particularly useful to geneticist s because parts of it can be used to introduce foreign DNA into the bacterial genome; it is a cloning vector .

2. Blunt-end ligation
A lab technique to join together two pieces of blunt-end DNA , such as an insert into a cloning vector , which requires the enzyme ligase because there are no single-stranded overhanging ends for the attachment to form more spontaneously, by itself.

3. Charon phage
A cloning vector made from the virus bacteriophage lambda that is used to clone DNA .

4. Cloning vector
A DNA molecule originating from a virus , a plasmid , or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self-replication; vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. Examples are plasmids, cosmid s, and yeast artificial chromosome s; vectors are often recombinant molecules containing DNA sequences from several sources.

5. Col E1
A plasmid which naturally occurs in some strains of the bacteria Escherichia coli. It codes for an E. coli-produced antibiotic called a colicin and immunity to its self-produced colicin (so that it doesn't unintentionally destroy itself). It is one of a number of such plasmids, each of which code for a different type of colicin. The plasmid is useful for making cloning vector s for making recombinant DNA molecules.

6. Complementary DNA cloning (cDNA cloning)
A lab technique where a double-stranded cDNA molecule (or dscDNA ) is inserted into a cloning vector (another DNA molecule which will continue to be capable of replication after insertion of foreign material), so that the gene encoded by the cDNA can be expressed (transcribed and used) or so many copies of the gene can be made.

7. Cos cells
Cell s from a monkey which have had gene s from the virus SV40 added to it. The genes are used by the virus to replicate its DNA . The cells are used by scientists as host cells in cloning experiments to make lots of gene clones of eukaryotic DNA molecules of interest, along with cloning vector s made from more parts of the SV40 genome and in which the eukaryotic DNA of interest is inserted.

8. Cosmid
Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into E. coli ; this permits cloning of larger DNA fragments (up to 45 kb) that can be introduced into bacterial hosts in plasmid vectors.

9. Expression vector
A cloning vector that contains the necessary regulatory sequences to allow transcription and translation of a cloned gene or genes.

10. Expression vector
A cloning vector which is able to replicate and transcribe clone DNA .