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Found Cosmid 5 times.

Displaying results 1 to 10.

1. Arrayed library
Individual primary recombinant clone s (hosted in phage , cosmid , YAC , or other vector ) that are placed in two-dimensional arrays in microtiter dishes. Each primary clone can be identified by the identity of the plate and the clone location (row and column) on that plate. Arrayed libraries of clones can be used for many applications, including screening for a specific gene or genomic region of interest as well as for physical mapping . Information gathered on individual clones from various genetic linkage and physical map analyses is entered into a relational database and used to construct physical and genetic linkage map s simultaneously; clone identifiers serve to interrelate the multilevel maps. Compare library , genomic library .

2. Cloning vector
A DNA molecule originating from a virus , a plasmid , or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self-replication; vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. Examples are plasmids, cosmid s, and yeast artificial chromosome s; vectors are often recombinant molecules containing DNA sequences from several sources.

3. Cosmid
Artificially constructed cloning vector containing the cos gene of phage lambda. cosmids can be packaged in lambda phage particles for infection into E. coli ; this permits cloning of larger DNA fragments (up to 45 kb) that can be introduced into bacterial hosts in plasmid vectors.

4. Recombinant DNA
Recombinant DNA is a fragment of DNA incorporated artificially into the DNA molecule of a suitable vector so that it can express itself many times. This way a large quantity of the DNA in question can be obtained. The DNA is usually one that contains genes of interest, such as interferon , insulin , or growth hormone . The DNA may also be intended to fix mutated genes causing diseases, such as hemophilia or sickle cell anemia . The vector could be plasmid s, bacteriophage s, and cosmid s (packaged plasmid DNA into a phage particle).

5. Yeast artificial chromosome (YAC)
A vector used to clone DNA fragments (up to 400 kb); it is constructed from the telomeric, centromeric, and replication origin sequence s needed for replication in yeast cells. Compare cloning vector , cosmid .

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