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Found Electrophoresis 23 times.

Displaying results 1 to 10.

1. Agarose (agarbiose)
This linear galactan is created by purifying agar ; when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion . A typical gel is about 1% agarose. Agarose is more porous than acrylamide and is sold in different grades; the lower its sulfate content, the more highly purified it is.

2. Agarose gel electrophoresis
A type of electrophoresis that uses a matrix of highly purified agar to separate large DNA and RNA molecules (generally around 20,000 nucleotide s in size).

3. Autoradiography (radioautography)
A technique that uses X-ray film to visualize radioactively labeled molecules or fragments of molecules; used in analyzing length and number of DNA fragments after they are separated by gel electrophoresis .

4. Capillary electrophoresis
A technique for separating compounds; a sample of a compound to be separated is placed in a capillary tube, which is then subjected to a high voltage current that separates its chemical components.

5. Chemical sequencing
A lab technique used to determine the sequence of nucleotide s in a DNA molecule. The DNA molecule is labeled with radioactive phosphorous (chemical element P), cut into fragments, and analyzed through electrophoresis .

6. Creatine phosphokinase (CPK, CK)
This is an enzyme from the muscles of the heart, skeletal muscles and the brain. It is released to blood after damage, after which serum level rises in 6 hours, peaks in 18 hours, returns to normal in 2 to 3 days. It is often measured to confirm the clinical diagnosis of myocardial infarction. The isoenzymes can be fractionated by electrophoresis as CPK1 also known as CPK-BB from the brain; CPK 2 also known as CPK-MB originated from myocardial (heart muscle) damage; and CPK3 also known as CPK-MM from the skeletal muscles. CPK is elevated in: * myocardial damage from infarction, defibrillation (cardioversion), and myocarditis * skeletal muscle damage including convulsion, intramuscular injection, malignant hyperthermia, and radomyolysis * brain trauma, cardiovascular accidents, electroconvulsive therapy, and tumors (Normal range of CPK is 30 - 300 IU/ml with an MB fraction less than 4%)

7. Dideoxy sequencing (Sanger sequencing)
A lab technique to find out the sequence of nucleotide base s in a DNA molecule, invented by Fred Sanger. The technique involves putting the DNA molecule and the components needed to replicate it into each of four test tubes, along with one of the four types of dideoxynucleotide s (ddA, ddT, ddC, ddG), which are molecules that cause the premature termination of replication at the base that they substitute for. The result is that each of the test tubes contains fragments of the DNA molecule that all end at the same base, but at different points on the molecule where the base occurs. The contents of the test tubes are then separated by size with gel electrophoresis (one gel well per test tube; four total wells); the smallest fragments will travel the farthest and the largest will travel the least far from the well. The sequence can then be determined from the picture of the finished gel by noting the sequence of the marks on the gel and from which well they came from.

8. Disc electrophoresis
Short for "discontinuous electrophoresis," it is a type of polyacrylamide gel electrophoresis . This electrophoresis method uses gels of two different concentrations of polyacrylamide (a synthetic polymer ), the one of lower concentration stacked on top of the one with higher concentration, in order to better resolve bands of whatever is being separated ( DNA , RNA , or protein ) that would otherwise be very close together.

9. DNA footprinting
A lab technique that uses gel electrophoresis to find out which parts of a DNA molecule have sites for protein s to attach to them. The technique compares DNA which has been in the presence of DNA binding proteins with DNA that is "pure."

10. DNase footprinting
A lab technique used to find out which segments of a DNA molecule are protected by DNA-binding protein s from attack by endonuclease enzyme s, which break down DNA into smaller fragments by cleaving its phosphodiester bond s. The technique does this with gel electrophoresis to find out which parts of the DNA have sites for proteins to attach to them, by comparing DNA which has been in the presence of DNA-binding proteins with DNA that is "pure."