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Found Hybridization 20 times.

Displaying results 1 to 10.

1. Colony hybridization
A genetics lab technique used to identify which colonies of bacteria on an agar plate contain a particular sequence of DNA or a particular gene . The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that each colony contributes a small smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labeled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the agar plate. This technique is often used in conjunction with experiments involving the making of genomic libraries.

2. Competition hybridization
A lab technique used to determine how similar two strands of single-stranded nucleic acid s are to each other by putting them with a third strand (called a standard) and observing how well they can bond with each other to become double-stranded (how well they hybridize ).

3. Cross-hybridization (cross hybridization)
The hydrogen bonding of a single-stranded DNA sequence that is partially but not entirely complementary to a single-stranded substrate . Often, this involves hybridizing a DNA probe for a specific DNA sequence to the homologous sequences of different species .

4. Denhardt's solution
A solution commonly used during probe hybridization s that involve filters (such as Southern, Northern, or Western blot s). The solution contains ficoll, bovine serum albumin, polyvinylpyrrolidone (PVP), and a high concentration of nonspecific DNA so the probe won't hybridize nonspecifically.

5. DNA fingerprint
The unique pattern of DNA fragments identified by Southern hybridization (using a probe that binds to a polymorphic region of DNA) or by polymerase chain reaction (using primer s flanking the polymorphic region).

6. DNA hybridization
A lab technique used to find out how closely related two or more separate strands of DNA from different species are to each other. The technique involves radioactive labeling.

7. DNA hybridization
The formation of hybrid DNA molecules that contain a strand of DNA from two different species. The number of complementary sequences in common in the two strands is an indication of the degree of relatedness of the species.

8. DNA-RNA hybridization
A type of hybridization . In this case, a strand of DNA is joined with a complementary strand of RNA to form a double-stranded molecule (or one which is partly double-stranded, if one of the original single strands is shorter than the other).

9. FISH (fluorescence in situ hybridization)
A physical mapping approach that uses fluorescent tags to detect hybridization of probe s with metaphase chromosome s and with the less-condensed somatic interphase chromatin.

10. Grunstein and Hogness method
The Grunstein and Hogness method is a hybridization lab technique in which whole, lysed (ruptured) bacterial colonies which have previously been blotted onto a nitrocellulose (or other) membrane are hybridized to a DNA probe .