BioScience Dictionary

 
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Found Phosphodiester bond 10 times.

Displaying results 1 to 10.

1. DNA ligation
The joining of two DNA strands by their ends with a phosphodiester bond .

2. DNase footprinting
A lab technique used to find out which segments of a DNA molecule are protected by DNA-binding protein s from attack by endonuclease enzyme s, which break down DNA into smaller fragments by cleaving its phosphodiester bond s. The technique does this with gel electrophoresis to find out which parts of the DNA have sites for proteins to attach to them, by comparing DNA which has been in the presence of DNA-binding proteins with DNA that is "pure."

3. DNase I
A type of endonuclease , or enzyme that breaks down DNA molecules at their phosphodiester bond s. It is found in large quantities in the pancreas.

4. DNase I hypersensitivity site (DNase I hypersensitive site)
A site on a DNA molecule that is especially prone to being cut apart by the endonuclease enzyme DNase I , which breaks down DNA into smaller fragments by cleaving phosphodiester bond s. These sites tend to be near active gene s, which are regularly transcribed.

5. DNase I sensitivity
The likelihood that a particular DNA segment will be attacked by the endonuclease enzyme DNase I , which breaks down DNA into smaller fragments by cleaving its phosphodiester bond s. DNA segments which contain active gene s that are regularly transcribed are more prone to being cut apart by DNase I than inactivated genes, because the inactivated genes are generally within condensed regions of the chromosome , while the active genes are in more open configurations for easy access for transcription.

6. EcoRI restriction enzyme
A commonly-used restriction enzyme ( enzyme which will cleave the phosphodiester bond s of DNA at specific nucleotide sequences) that came from the bacteria Escherichia coli and recognizes the sequence GAATTC. The enzyme will make a staggered cut of the double-stranded DNA molecule by cutting between the G and A on both strands.

7. Endonuclease
An enzyme that cleaves its nucleic acid substrate at internal sites (the phosphodiester bonds) in the nucleotide sequence.

8. Endonuclease S1
A nuclease enzyme from the fungus Aspergillus oryzae which cuts the phosphodiester bond s between nucleotide s in single-stranded DNA or RNA , producing individual nucleotide molecules.

9. Esterase
An enzyme that breaks ester linkages, especially the ones found in nucleic acid s ( phosphodiester bond s) and lipid s.

10. Phosphodiester bond
The covalent bond that attaches a phosphate group to a molecule by way of an oxygen atom (which, out of two possible bonds, is bonded to the phosphate group on one side and the rest of the molecule on the other). This is the type of bond that links the nucleotide subunits together to make a DNA or RNA molecule.