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Found Probe 30 times.

Displaying results 1 to 10.

1. Anneal
* The sustained heating of a material such as steel or glass at a specific high temperature, followed by gradual cooling; this is done to eliminate weakness or to produce other qualities. * The pairing of complementary DNA or RNA sequence s, via hydrogen bonding , to form a double-stranded molecule. Most often used to describe the binding of a short primer or probe .

2. Chemiluminescent labeling
A technique of labeling DNA strands; two different DNA probe s emit light when they come together in the same region of a gene .

3. Chromogenic label
A molecule which can be used as a label to a biological probe molecule because it forms a colored compound when the probe molecule is attached to its target.

4. Colony hybridization
A genetics lab technique used to identify which colonies of bacteria on an agar plate contain a particular sequence of DNA or a particular gene . The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that each colony contributes a small smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labeled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the agar plate. This technique is often used in conjunction with experiments involving the making of genomic libraries.

5. Complementary DNA (cDNA)
DNA that is synthesized from a messenger RNA template; the single- stranded form is often used as a probe in physical mapping .

6. Complementary DNA library (cDNA library)
A collection of all of the mRNA molecules present in a cell or organism , all turned into cDNA molecules with the enzyme reverse transcriptase , then inserted into vector s (other DNA molecules which can continue to replicate after addition of foreign DNA). The library can then be probe d for the specific cDNA (and thus mRNA) of interest.

7. Cross-hybridization (cross hybridization)
The hydrogen bonding of a single-stranded DNA sequence that is partially but not entirely complementary to a single-stranded substrate . Often, this involves hybridizing a DNA probe for a specific DNA sequence to the homologous sequences of different species .

8. Cryoprobe
A surgical instrument used to apply extreme cold to tissues.

9. Czapek's medium
An agar medium used to grow fungi and soil bacteria which feed on decaying organic material (called saprobe s). The medium contents include sugars, water, and salt.

10. Denhardt's solution
A solution commonly used during probe hybridization s that involve filters (such as Southern, Northern, or Western blot s). The solution contains ficoll, bovine serum albumin, polyvinylpyrrolidone (PVP), and a high concentration of nonspecific DNA so the probe won't hybridize nonspecifically.

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