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Found Restriction 28 times.

Displaying results 1 to 10.

1. BglII
A restriction enzyme that cuts DNA in a staggered manner wherever it finds the sequence "AGATCT."

2. Directional cloning
DNA insert and vector molecules are digested with two different restriction enzyme s to create noncomplementary sticky end s at either end of each restriction fragment. This allows the insert to be ligated to the vector in a specific orientation and prevents the vector from recircularizing.

3. Double digestion
Treating DNA with two different restriction enzyme s, or enzyme s which will cleave DNA at specific nucleotide sequences.

4. EcoRI methylase
An enzyme that will methylate (attach a methyl group (-CH3 group) to the nitrogenous base adenine on DNA molecules) the cutting site (the sequence GAATTC) of the EcoRI restriction enzyme (an enzyme which will cleave DNA at specific nucleotide sequences).

5. EcoRI restriction enzyme
A commonly-used restriction enzyme ( enzyme which will cleave the phosphodiester bond s of DNA at specific nucleotide sequences) that came from the bacteria Escherichia coli and recognizes the sequence GAATTC. The enzyme will make a staggered cut of the double-stranded DNA molecule by cutting between the G and A on both strands.

6. Frozen section
A frozen section is a technique to prepare tissue for microscopic examination. It involves freezing the tissue and cutting it in a block of ice. This way samples are ready within a few minutes. However, there are certain restrictions in making medical diagnosis based on frozen sections.

7. HindII (Hind II, Hind II restriction enzyme)
A restriction enzyme which cuts DNA wherever it sees the following nucleotide sequence: 5' G T Py Pu A C 3' and 3' C A Pu Py G T 5' . The enzyme cuts the DNA between the Py and Pu on both strands. The Hind II enzyme was originally found in the bacterium Hemophilus influenzae.

8. HindIII (Hind III, Hind III restriction enzyme)
A restriction enzyme which cuts DNA wherever it sees the following nucleotide sequence: 5' AAGCTT 3' and 3' TTCGAA 5' . The enzyme cuts the DNA between the two A's on each strand (it makes a staggered cut). The Hind III enzyme was originally found in the bacterium Hemophilus influenzae.

9. Host restriction-modification
A bacterial system where the bacterium is able to destroy invading DNA from a bacteriophage ( virus which infects bacteria) while at the same time preventing the destruction of their own DNA. The phage DNA is cleaved by a restriction enzyme made by the bacterium; the bacterial DNA is modified (usually with methylation ) so that the enzyme won't destroy it.

10. Immortalized cells
Cell s which have been transformed so that they are able to grow and reproduce without restriction (given ample nutrients).


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