BioScience Dictionary

 
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Found Restriction enzyme 15 times.

Displaying results 1 to 10.

1. BglII
A restriction enzyme that cuts DNA in a staggered manner wherever it finds the sequence "AGATCT."

2. Double digestion
Treating DNA with two different restriction enzyme s, or enzyme s which will cleave DNA at specific nucleotide sequences.

3. EcoRI methylase
An enzyme that will methylate (attach a methyl group (-CH3 group) to the nitrogenous base adenine on DNA molecules) the cutting site (the sequence GAATTC) of the EcoRI restriction enzyme (an enzyme which will cleave DNA at specific nucleotide sequences).

4. EcoRI restriction enzyme
A commonly-used restriction enzyme ( enzyme which will cleave the phosphodiester bond s of DNA at specific nucleotide sequences) that came from the bacteria Escherichia coli and recognizes the sequence GAATTC. The enzyme will make a staggered cut of the double-stranded DNA molecule by cutting between the G and A on both strands.

5. HindII (Hind II, Hind II restriction enzyme)
A restriction enzyme which cuts DNA wherever it sees the following nucleotide sequence: 5' G T Py Pu A C 3' and 3' C A Pu Py G T 5' . The enzyme cuts the DNA between the Py and Pu on both strands. The Hind II enzyme was originally found in the bacterium Hemophilus influenzae.

6. HindIII (Hind III, Hind III restriction enzyme)
A restriction enzyme which cuts DNA wherever it sees the following nucleotide sequence: 5' AAGCTT 3' and 3' TTCGAA 5' . The enzyme cuts the DNA between the two A's on each strand (it makes a staggered cut). The Hind III enzyme was originally found in the bacterium Hemophilus influenzae.

7. Macrorestriction map
Map depicting the order of and distance between sites at which restriction enzyme s cleave chromosome s.

8. Marker
An identifiable physical location on a chromosome (e.g., restriction enzyme cutting site , gene ) whose inheritance can be monitored. Markers can be expressed regions of DNA (genes) or some segment of DNA with no known coding function but whose pattern of inheritance can be determined. See RFLP, restriction fragment length polymorphism .

9. Physical map
A map of the locations of identifiable landmarks on DNA (e.g., restriction enzyme cutting sites, gene s), regardless of inheritance. Distance is measured in base pair s. For the human genome , the lowest-resolution physical map is the banding patterns on the 24 different chromosome s; the highest-resolution map would be the complete nucleotide sequence of the chromosomes.

10. Recombinant DNA molecules
New combinations of DNA fragments formed by cutting DNA segments from two sources with restriction enzyme and then joining the fragments together with DNA ligase. Interspecies transfer of genes usually through a vector such as a virus or plasmid.