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Found Vector 46 times.

Displaying results 1 to 10.

1. Adaptors
Short synthetic oligonucleotide strands that have one sticky end and one blunt end; the blunt ends join to the blunt end of a DNA fragment, forming a new fragment with two sticky ends that can be more easily spliced into a vector .

2. Alpha viruses
These are the major virus es in the Togavirus group and include eastern and western equine encephalitis viruses, the Sindbis virus and the Semliki Forest virus. The last two viruses are being researched as vector s for expressing heterologous gene s.

3. Amphotropic packaging cell lines, amphotropic virus
Clonal entities that express genes or act as viral vector s that infect cell lines to stably infect and then express genes of choice.

4. Arrayed library
Individual primary recombinant clone s (hosted in phage , cosmid , YAC , or other vector ) that are placed in two-dimensional arrays in microtiter dishes. Each primary clone can be identified by the identity of the plate and the clone location (row and column) on that plate. Arrayed libraries of clones can be used for many applications, including screening for a specific gene or genomic region of interest as well as for physical mapping . Information gathered on individual clones from various genetic linkage and physical map analyses is entered into a relational database and used to construct physical and genetic linkage map s simultaneously; clone identifiers serve to interrelate the multilevel maps. Compare library , genomic library .

5. Bacteriophage lambda
A bacteriophage (a virus which infects bacteria ) that infects E. coli . It has a complex set of regulatory mechanisms to determine whether it will quietly insert its DNA into the bacterial genome to become dormant and to be reproduced whenever the bacterium reproduces (to lysogenize ), or whether it will hijack the bacterium's cellular machinery to reproduce itself and prepare to infect more bacteria, causing the bacterium to self-destruct shortly after infection (to lyse ). Lambda is particularly useful to geneticist s because parts of it can be used to introduce foreign DNA into the bacterial genome; it is a cloning vector .

6. Blunt-end ligation
A lab technique to join together two pieces of blunt-end DNA , such as an insert into a cloning vector , which requires the enzyme ligase because there are no single-stranded overhanging ends for the attachment to form more spontaneously, by itself.

7. Charon phage
A cloning vector made from the virus bacteriophage lambda that is used to clone DNA .

8. Cloning vector
A DNA molecule originating from a virus , a plasmid , or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self-replication; vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. Examples are plasmids, cosmid s, and yeast artificial chromosome s; vectors are often recombinant molecules containing DNA sequences from several sources.

9. Col E1
A plasmid which naturally occurs in some strains of the bacteria Escherichia coli. It codes for an E. coli-produced antibiotic called a colicin and immunity to its self-produced colicin (so that it doesn't unintentionally destroy itself). It is one of a number of such plasmids, each of which code for a different type of colicin. The plasmid is useful for making cloning vector s for making recombinant DNA molecules.

10. Complementary DNA cloning (cDNA cloning)
A lab technique where a double-stranded cDNA molecule (or dscDNA ) is inserted into a cloning vector (another DNA molecule which will continue to be capable of replication after insertion of foreign material), so that the gene encoded by the cDNA can be expressed (transcribed and used) or so many copies of the gene can be made.

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