Beta-actin

Actin, beta
	; PDB rendering based on 1atn.
Available structures
PDB	Ortholog search: PDBe, RCSB
List of PDB id codes
	3BYH, 3D2U, 3LUE
Identifiers
Symbols	ACTB; BRWS1; PS1TP5BP1
External IDs	OMIM: 102630 MGI: 87904 HomoloGene: 110648 GeneCards: ACTB Gene
Gene Ontology
Molecular function	• structural constituent of cytoskeleton; • protein binding; • ATP binding; • kinesin binding; • protein kinase binding; • Tat protein binding; • nitric-oxide synthase binding;
Cellular component	• cytoplasm; • cytosol; • cytoskeleton; • postsynaptic density; • axon; • ribonucleoprotein complex; • cortical cytoskeleton; • NuA4 histone acetyltransferase complex; • extracellular vesicular exosome; • MLL5-L complex;
Biological process	• protein folding; • cellular component movement; • axon guidance; • blood coagulation; • cellular membrane organization; • cell junction assembly; • adherens junction organization; • Fc-gamma receptor signaling pathway involved in phagocytosis; • cellular protein metabolic process; • innate immune response; • cell-cell junction organization; • 'de novo' posttranslational protein folding;
	Sources: Amigo / QuickGO
RNA expression pattern
	More reference expression data
Orthologs
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Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins¹ that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.²

Interactions

Beta-actin has been shown to interact with SPTBN2.³⁴ In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components.

Clinical relevance

Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma.⁵

Applications

Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 42 kD.

References

^ Hanukoglu I, Tanese N, Fuchs E (February 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions". J. Mol. Biol. 163 (4): 673–8. PMID 6842590.
^ "Entrez Gene: ACTB actin, beta".
^ Mao B, Wu W, Li Y, Hoppe D, Stannek P, Glinka A, Niehrs C (May 2001). "LDL-receptor-related protein 6 is a receptor for Dickkopf proteins". Nature 411 (6835): 321–5. doi:10.1038/35077108. PMID 11357136.
^ Holleran EA, Ligon LA, Tokito M, Stankewich MC, Morrow JS, Holzbaur EL (September 2001). "beta III spectrin binds to the Arp1 subunit of dynactin". J. Biol. Chem. 276 (39): 36598–605. doi:10.1074/jbc.M104838200. PMID 11461920.
^ Lohr JG, Stojanov P, Lawrence MS, Auclair D, Chapuy B, Sougnez C, Cruz-Gordillo P, Knoechel B, Asmann YW, Slager SL, Novak AJ, Dogan A, Ansell SM, Link BK, Zou L, Gould J, Saksena G, Stransky N, Rangel-Escareño C, Fernandez-Lopez JC, Hidalgo-Miranda A, Melendez-Zajgla J, Hernández-Lemus E, Schwarz-Cruz y Celis A, Imaz-Rosshandler I, Ojesina AI, Jung J, Pedamallu CS, Lander ES, Habermann TM, Cerhan JR, Shipp MA, Getz G, Golub TR (March 2012). "Discovery and prioritization of somatic mutations in diffuse large B-cell lymphoma (DLBCL) by whole-exome sequencing". Proc. Natl. Acad. Sci. U.S.A. 109 (10): 3879–84. doi:10.1073/pnas.1121343109. PMC 3309757. PMID 22343534.

Beta-actin

Contents

Interactions

Clinical relevance

Applications

References

Further reading

See also