Damodaran M. Vasudevan

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

D M Vasudevan

Professor Damodaran M Vasudevan was the Dean of the College of Medicine at Amrita Vishwa Vidyapeetham and the Principal of the Amrita Institute of Medical Sciences and Research, Kochi, India. After retirement on 31.12.2009, he still continues as the Head of PG Programs and Research, at Amrita Institute of Medical Sciences and Research, Kochi.

After completing his Bachelor of Medicine and Surgery, he received his MD in Biochemistry from All India Institute of Medical Sciences in 1971. Subsequently, he became a Fellow of the Royal College of Pathologists in 1994. He is an authority in allergy and immunology and has also worked extensively on cancer too.

He has been the Principal of Government Medical College Thrissur. After his retirement from there, he joined the Kasturba Medical College, Mangalore and the Sikkim Manipal Institute of Medical Sciences as the Dean in 2000. From 2002 he has been in his present position.

Vasudevan, an elected fellow of the National Academy of Medical Sciences,[1] received the prestigious Dr. B. C. Roy Award of the Medical Council of India from the hands of the then Hon'ble President of India in 1992 for excellence in the medical teaching profession. Dr Vasudevan will be fondly remembered by his students, as a committed teacher, guide and scholar in Biochemistry. He has authored the Textbook of Biochemistry for Medical Students, first edition was published in 1995, the eight edition in 2016 and the 9th edition in 2019 (2). The book is now well known internationally. The Spanish and Slovak editions are already in print. The textbook is widely circulated in 23 countries of the world. Dr Vasudevan also authored textbooks for Dental students, paramedical students. He has guided 30 students for their PhD work. He has also published more than 240 papers peer reviewed scientific journals.

Summary of Research work done under the guidance of Dr. Vasudevan

Cancer and Viruses : Out of about 4000 new cancer patients registered every year at the Regional Cancer Centre, Trivandrum, 27% are affected with oral cancer, 14% with cer-vical cancer and 7% with breast cancer (3). Although the incidence is de-creasing, oral cancer is still is an important health problem.

There are only scanty reports available with regard to the relationship, if any, of oral cancers with viruses. Therefore, sero epidemiological studies on the prevalence of anti herpes simplex virus (HSV) antibodies in circula-tion of persons using serum samples from 919 oral cancer patients, 586 cer-vical cancer patients, 890 patients with other unrelated cancers and 650 normal healthy persons were carried out (4, 5). Anti-HSV-1 antibodies were seen in 71% of oral cancer patients, as against 52% in normal persons (6). Moreover, the titre values of antivirus antibodies were very high in oral can-cer group. No significant association between oral cancer with HSV-2 or ad-enovirus was noticed, even though HSV-2 and adenovirus infections are al-so very common in this region. Biopsies from 175 patients suffering from carcinoma of oral cavity were examined for the presence of HSV-1 antigen, using immuno fluorescence and immuno peroxidase techniques (6). 77% of biopsies showed significant fluorescence, while 71% showed positivity with peroxidase staining. Normal oral biopsies showed 20-30% positivity.

Anti-HSV-2 antibodies were seen in significantly higher percentage of cases and of very high titres in sera of cervical cancer patients. All other pa-tient controls, except those with dysplasia and carcinoma in situ, revealed a prevalence of anti-HSV-2 antibodies comparable to normal females (7). Indi-rect immuno fluorescence technique was employed to detect HSV-2 anti-gens in tumour biopsies from 215 patients with carcinoma of uterine cervix, when 70% revealed brilliant fluorescence, while only 27% of normal cervix specimen and 58% of cervical dysplasia showed positive fluorescence (8). HSV-2 was shown to be associated with cervical cancer by immunofluores-cence and molecular biology techniques (9).

Since HSV-1 antibodies were more in oral cancer patients, and the HSV-1 antigens were present on the oral cancer cells, it was worthwhile to test whether the HSV genome was present in oral cancer cellular DNA. Thus, DNA from cancer specimens were extracted, denatured, precipitated on nitrocellulose membrane and hybridized with radio active nick-translated cloned HSV probes. The HSV-1 middle segment (M-A fragment) probe hy-bridized with the DNA from 64% oral cancer tissues, as against 20% normal tissues. HSV-1 E and K fragments hybridized with the DNA from 56% oral cancer tissues, while only 8% DNA samples from normal tissues showed hybridization with this fragment (10). In-situ hybridisation studies, using ECoRI D and I fragments of HSV-1 showed 60% positivity in oral cancer tis-sues, but only 12% of normal tissues. Human Papilloma Virus (HPV-16) was seen to be hybridized with 10% oral cancer and 37% cervical cancer speci-mens (11).

Further work was carried out on the probable association of human herpes virus type 6 (HHV-6) in cancer patients. The very first study in India with regard to the sero prevalence of anti HHV-6 antibodies in normal as well as in cancer patients was published by Dr. Vasudevan (12). Smears were prepared with Molt-3 cells infected with HHV-6, incubated with sera from pa-tients, washed and immunoperoxidase staining was done. In normal con-trols, 76% was positive for anti-HHV-6 antibodies, and titres ranged from 10-160 with a mean of 47. In oral cancer cases, 96% patients were positive, and titres ranged from 80-10250 with mean of 2042 (13). The anti-HHV-6 anti-bodies were also seen to be significantly elevated in Hodgkin’s lymphoma, and leukemia; but not elevated in Non-Hodgkin’s lymphoma, cervical can-cers and breast cancers (13). All these findings have been incorporated into a review article (14).

Cancer immunology: The cellular immunity in patients with solid tumour was assessed, and the correlation in prognosis was tested. The circulating T cells were found to be lowered in cancer patients (15). When T cell subsets were further analysed, the oral cancer patients showed a significant increase in the proportion of TG cells and a significant reduction in TM cells. The discrepancy in the TG sub-set was more evident in the early stages of the disease, while TM cell defi-ciency was apparent only in advanced stages (16). Total rosette forming cells and high affinity rosette forming cells (HRFC) were enumerated in 534 patients with carcinoma of oral cavity, uterine cervix and breast cancer, and compared with controls (16). A decrease in absolute peripheral lymphocyte count was observed in cancer patients (17). Generally speaking, the depres-sion in immunity is more pronounced in patients with poor prognosis.

Serum and tissue immunoglobulin levels were evaluated in solid can-cers (18). Immunoglobulins (IgG, IgM and IgA) were shown to be present in appreciable quantities in the tissues of oral cancer (19) and these could be eluted from tissues (20). Various methods were standardized to assess the circulating immune complexes (21). Elevations of circulating immune com-plexes were found in oral cancer, breast cancer and gynecological tumours (22). About 81% of the samples from oral cancer patients and 88% of the samples from cervical cancer patients showed highly elevated immune com-plex levels (23). The level of immune complexes in the sera of patients who had a clinical cure decreased significantly, whereas it remained elevated in patients who had residual lesions (24).

Attempts were made to isolate the tumour associated antigen from human oral cancer tissues (25). A saline extract from oral cancer biopsy specimens was fractionated by Sephadex column chromatography, and one particular fraction contained the specific antigen, which was reacting with specific antiserum. This particular fraction was absent in the normal tissues. Further, the immune complexes were isolated by column chromatography. This antigen was not cross reacting with carcino-embryonic antigen (CEA) or alpha feto protein (AFP), but was reacting with humoral antibodies (26).

The human uterine cervix carcinoma cells were found to possess in-creased surface negative charges, due to the increase in sialic acid content on the surface of cancer cells, as compared to normal counterparts (27). Neuraminidase liberated more N-acetyl-neuraminic acid from cervical carci-noma cells than from normal cells. Autologous and homologous lympho-cytes from carcinoma cervix patients had a lytic effect on carcinoma cervix cells; and the cytolysis was increased about two-fold when the malignant cells were treated with neuraminidase. Homologous lymphocytes from healthy donors had comparatively little cytolytic action on malignant cells. These results suggest the possibility that in the case of cervical carcinoma, sialic acid containing moieties partially mask tumour specific antigens on cell surface and prevent their recognition by immunologically competent cells of the host (27). Changes in the glycosaminoglycan composition of human oral cancer tissue were also noticed when compared with normal counter-parts (28). An absolute increase in the amounts of hyaluronic acid, chon-droitin sulfate and dermatan sulfate was observed in oral cancer tissue (28).

Alterations in the surface components of cancer cells were also stud-ied by tissue binding patterns of lectins. Since lectins have specific carbo-hydrate binding properties, these were found to attach specifically on various tissues. In normal sections, lectins showed a uniformed staining in base-ment membrane and in keratinized area; while in squamous cell carcinoma, the lectin binding was irregular (29, 30). The difference in nature and inten-sity of binding of lectin clearly showed development of new antigens during the malignant transformation. Similarly Jackfruit lectin showed increased affinity to neoplastic cells in breast cancer specimens (31) as well as in car-cinoma of uterine cervix (32). Further, various lectins were shown to have immunopotentiation properties (33, 34).

To define the base level functions of immunology, various immuno-logical parameters during tumour development in murine models were stud-ied (35). In the Dalton’s lymphoma bearing animals, the NK activity reached a peak on the 12th day, ADCC peak activity was seen on the 5th day, and the cytotoxic T lymphocytes showed maximum activity on the 10th day of tumor inoculation (36). In the advanced stage of tumor growth, the NK cell activity was shown to be declined. Pretreatment of these deficient effector cells with Interleukin-2 resulted in augmentation of NK cell activity. Cytotoxic T lym-phocytes (CTL) generated in vitro using mixed lymphocyte target cell culture were further expanded by treating with Interleukin-2 (37). These activated CTL showed enhanced cytotoxicity against syngeneic target tumor cells. The antitumor efficacy of the activated CTL in vivo was studied by using an adoptive immunotherapy model. The growth of tumor was significantly sup-pressed by adoptive transfer of activated CTL. Similar results were repeated in human gastric carcinoma cases also (38, 39, 40).

Attempts were done for physical isolation of active cytotoxic T lympho-cytes from mice immunized with syngeneic EL4 tumour cells (41). Fractiona-tion on a BSA density gradient yielded a low density (less than 1.08 g/ cm3) spleen cell population with a 10-fold increased cytotoxic activity. A similar increase was observed after pre-incubation of immune spleen cells for 24 hr at 37O C (42). Pre-incubation followed by BSA gradient separation resulted in a cumulative 100 fold increase in cytotoxicity. These active cells were shown to be T cells, as they are abolished by anti-theta serum. By a combi-nation of successive purification steps of pre-incubation for 24 hours, then density gradient separation of low density lymphocytes and finally by velocity sedimentation technique, thereby selecting the larger sized lymphocytes could result in 100,000 times enrichment of cytolytic T lymphocytes from the original spleen population (43, 44). These purified populations of lympho-cytes were shown to be biologically effective in suppressing the tumor growth in vivo in syngeneic animals. Selection of specifically active lympho-cytes could also be generated by co-cultivation of immune lymphocytes with irradiated syngeneic tumor cells (44).

Treatment adjuvants: Levamisole appears to have beneficial effect in prolonging the disease free interval in human oral cancer cases (45). Levamisole also hastened the res-toration of leukopenia observed after radiotherapy (46, 47). Tumor bearing mice exhibit a specific receptor in the ascites fluid which binds with the ac-tive component isolated from Iscador from Viscum album (48). Iscador was found to be cytotoxic to Dalton’s lymphoma ascites cells, Ehrlich ascites cells and lung fibroblasts cells, hamster ovary cells and human nasopharyngeal carcinoma cells (49). Moreover administration of Iscador was found to re-duce ascites and solid tumors (50). The active component has been isolated from the Iscador (51).

Radioprotective agents: Mercapto propionyl glycine (MPG) has no noticeable effect on the blood cells; but it definitely provides a significant protection to them during radio-therapy (52, 53). MPG was also found to be an effective chemoprotector It reduced the leucopenia induced by cyclophosphamide in mice. MPG treat-ment enhanced the survival of mice against cyclophosphamide induced mortality (54). Protective effect of MPG against the side effects of cyclophos-phamide was studied in normal as well as tumor bearing mice (55). Experi-ments with different doses of MPG and cyclophosphamide indicated that MPG repairs the cyclophosphamide induced lethal damages, while retaining the anti-cancer property of cyclophosphamide (55, 56). When MPG is em-ployed, higher doses of anti-cancer drug could be used with an effective cure of cancer (57). The immunological aspects of human cancers are summarised in a review article (58).

Alcohol and liver: Ethanol increases the rate of generation of free radicals, decreases antioxi-dant levels, and potentiates oxidative stress. Several biochemical and hema-tological tests, such as GGT, AST and HDL-C content of serum are estab-lished markers of alcohol intake. Carbohydrate deficient transferrin (CDT), β-hexosaminidase, acetaldehyde adducts and the urinary ratio of serotonin metabolites, 5-hydroxytryptophol and 5-hydroxyindoleacetic acid are new markers (59, 60). Long term effect of alcoholism on adhesion molecules (61), angionesis (62), fibrosis (63) and liver cell death (64) are also studied. The implications are reviewed (65, 66, 67, 68). Ethanol treatment enhanced lipid peroxidation significantly. Degree of oxidative injury was similar in patients with non-alcoholic liver disease and in moderate drinkers; while significantly higher in heavy drinkers (69). Ethanol ingestion perturbs the antioxidant sys-tem in a dose and time dependent manner (70, 71). Ethanol induced oxida-tive stress could be protected by silymarin (72) and lecithin (73) and tocoph-erol (74). These findings are reviewed (75, 76, 77).

Alcohol and brain': Chronic ethanol administration is able to induce an oxidative stress in the central nervous system (78). Ascorbic acid level; and activities of alkaline phosphatase and aspartate transaminase in the brain are dependent on the concentration of ethanol exposure. Alcohol reinforces drinking by producing a mild euphoria. The reinforcing effect is mediated by neurochemical sys-tems (79). Alcohol affects brain function by interfering with gamma-aminobutyric acid (GABA) and other neurotransmitters (80). The GABA concentration was elevated, while isoleucine and leucine levels reduced significantly in ALD patients compared to the control subjects. Methionine and phenylalanine levels elevated and valine content reduced significantly in ALD patients compared to other two groups, and GABA level was signifi-cantly correlated with methionine and phenylalanine. Plasma concentration of lysine was significantly reduced in both groups of liver disease patients compared to the control group (81). Glycine and tyrosine levels were reduced significantly in NAFLD patients compared to other two groups and were sig-nificantly correlated with GABA (82). This study indicated that alcoholic liver disease presented a more deranged plasma amino acid pattern than nonal-coholic, and the amino acid imbalances. (83, 84). The free radical scaveng-ing properties of grape skin and grape flesh (contains natural antioxidants like polyphenols) was determined in vitro and for in vivo studies. Results in-dicate that grape skin extracts showed significant radical scavenging proper-ties (85). There was also significantly alteration of serum cytokines (86, 87). Reviews are published on this subject (88, 89).

Alcohol and Reproductive System: Chronic ethanol administration in rats caused drastic reduction in the body weight and testis weight. Simultaneously there was increase in oxidative stress and decrease in steroidogenesis (90). Chronic ethanol administration resulted in high oxidative stress in the testes either due to increased extent of lipid peroxidation or due to decreased antioxidant defenses, and thereby induces germ cell apoptosis leading to testicular atrophy (92). In rats, etha-nol-induced oxidative stress and decreased steroidogenesis can be reversed by treatment with lecithin (90), alpha tocopherol (93, 94) ascorbic acid (95) or ornithine-aspartate (96). Patients with alcohol abuse had significantly low plasma testosterone, low LH, low FSH and increased oxidative stress (97).

Ocimum Sanctum: Ocimum sanctum, the sa¬cred 'Tulsi' finds diverse uses. The study showed antimicrobial activity of alcoholic and aqueous extracts of Ocimum sanctum on enteric pathogens such as E.coli, Klebsiella, Proteus, Salmonella, Shigel-la, Pseudomonas, Vibrio cholerae and Staphylococcus aureus. Antifungal activity of Ocimum extract was also shown against Candida albicans (98). Ocimum sanctum has significant ability to scavenge free radicals (99, 100). Hypolipidemic efficacy of Ocimum sanctum in the prevention of atherogene-sis in male albino rabbits was also shown (101). These findings are reviewed (102).

Studies on Coconut oil: In recent decades, the reputation of coconut oil has been clouded by the propaganda about its relation with atherosclerosis and heart attacks. This mis-conception arose because, it was known that saturated fatty acids (SFA) increase cholesterol and will adversely affect the heart. Since coconut oil contains saturated fatty acids, it was assumed that coconut oil is also bad to heart. But it has been shown that coconut oil contains medium chain fatty acids (MCFA). Later, it has been proved that the metabolism of MCFA is drastically different from SFA. Our studies on animals showed that coconut oil intake did not cause hypercholesterolemia. The lipoprotein levels and cholesterol levels are the same in sunflower fed animals and in coconut oil fed animals (103). Further, serum samples were analysed from 302 normal healthy persons, out of which 152 were consuming coconut oil and 150 were using sunflower oil for the past 2 years or more. There were no statistically significant differences in the cholesterol, HDL or LDL levels in coconut oil consuming population ver-sus sunflower oil consuming population (104).

It is known that LDL particles are more atherogenic. Study was con-ducted to assess LDL particles among normal subjects consuming either co-conut oil or sunflower oil. The proportion of healthy coconut oil consumers, with low LDL-cholesterol was high compared to the sunflower oil users (48% vs. 38%). Our studies showed no superiority of sunflower oil over coconut oil in reducing the LDL cholesterol in blood (105). In a parallel study, compari-son was done on the lipid profile and antioxidant enzymes of normal and di-abetic subjects consuming coconut oil and sunflower oil. Total glutathione and glutathione peroxidase were measured in erythrocytes and superoxide dismutase in serum. Though oxidative stress parameters were high in dia-betic subjects compared to controls, no pronounced changes for these pa-rameters were observed between the subgroups (coconut oil vs. sunflower oil) (106). Most importantly, lipid profile or oxidative stress parameters did not show significant changes between coconut oil and sunflower oil groups.

The fatty acid composition of the plaques taken from diseased arteries. A total of 71 samples of plaques were analysed, of which 48 persons were using coconut oil and 23 persons were using sunflower oil routinely (106). Plaques did not contain significant amounts of Lauric acid or Myristic acid (fatty acids from coconut oil). Instead, palmitic acid and stearic acid (long chain saturated fatty acids) were the main ingredients of these plaques. Fatty acid content of plaques from coconut oil consuming group and sunflower consuming group were the same. This clearly shows that coconut oil does not have an effect to produce plaque or heart disease.

As continuation of these findings, a large scale randomized case con-trol study comparing the impact of coconut oil versus sunflower oil as a cook-ing medium on the cardiovascular events and risk factors was done. Two hundred patients with heart diseases were enrolled for a 2 years follow up. All of them were getting their usual drugs. One group (100 patients) was us-ing only coconut oil, while the other 100 patients were using only sunflower oil. Clinical and biochemical parameters (total 32 parameters) were studied at 6 monthly intervals for 2 years. At the end of the 2 year study, all conven-tional atherosclerotic risk factors such as total cholesterol, LDL cholesterol, triglycerides etc were remained the same in both groups Antioxidant markers (lipid peroxidase, glutathione reductase, glutathione-S-transferase, superox-ide dismutase, catalase) were again shown to be the same in both groups. High sensitive CRP (hsCRP) (an important inflammatory marker for future heart attack) was also found to be the same level in both coconut oil users and sunflower oil users. The clinical outcome like death, Myocardial infarc-tion and Stroke were not different in both groups (107, 108). These findings on coconut oil studies are summarised (109, 110).

Inborn errors of metabolism: Screening tests and thin layer chromatography were done for detection of organic acidurias. Identification and quantitation of organic acids in urine and quantification of amino acids in blood were done by high performance liquid chromatography. Out of 420 patients, 45 patients (10.7%) were found to have organic acidurias. 15 cases of methylmalonic aciduria, 16 cases of propionic aciduria, 13 cases of MSUD, and one case of isovaleric aciduria were diagnosed. Since organic aciduria may cause irreversible brain dam-age if not treated, selective screening amongst severely ill children is rec-ommended (111). MSUD is caused by mutations in the BCKDHA, BCKDHB and DBT genes, which encode for the E1 alpha, beta and E2 subunits of the branched chain alpha keto acid dehydrogenase complex respectively. MSUD patients were studied for mutation analysis. Four novel mutations were identified. Notably, patients harbouring these mutations were non-responsive to thiamine supplementation and other treatment regimens (112). An updated review of organic aciduria is published (113. 114).


References

  1. ^ "List of Fellows - NAMS" (PDF). National Academy of Medical Sciences. 2016. Retrieved 19 March 2016.

2. D.M. Vasudevan, Sreekumari S and Kannan Vaidyanathan, Textbook of Biochemistry for Medical Students, 9th ed, Jaypee Brothers Medical Publishers, New Delhi, India, 20019.

3. A statistical analysis of Cancers registered at the Regional Cancer Centre, Trivandrum, during 1977 to 1980: T.K. Padmanabhan and D.M.Vasudevan: Indian J. Cancer, 19, 189-196, 1982. PubMed PMID No.7152545

4. Prevalence of Antibodies against Herpes Simplex and Adenoviruses in patients with Cervical and Oral Cancer, a preliminary report: T.V. Kumari, T. Shanmugam, B. Prabha and D.M.Vasudevan: Indian J. Medical Research 75, 590-592, 1982. PubMed, PMID No.6286474

5.Detection of Antibodies against Herpes Simplex Virus in patients with Oral Cancers: T.V. Kumari, H. Thankamani, B. Prabha, V.K. Sasidharan and D.M.Vasudevan: Indian J.Cancer, 21, 137-140, 1984 1985. PubMed PMID No. 6100592

6.Demonstration of HSV-1 Antigen in Patients with Oral Cancer by Immunofluorescence and Immunoperoxidase techniques: T.V. Kumari, D.M.Vasudevan, R. Ankathil, P. Remani and T. Vijayakumar: J. Experimental Pathology., 3, 75-86, 1987. PubMed PMID No 2824733

7.Serum Antibodies to Herpes Simplex Virus Type-2 in Carcinoma of the Uterine Cervix: V. Thankamani and D.M.Vasudevan: Indian. J. Medical Microbiology., 5, 297-304, 1987. 8.Papilloma Virus in Cervical Carcinoma: Detection of Viral Antigen in Cancer Cells: V. Thankamani, T.V. Kumari and D.M.Vasudevan: Journal of Experimental Pathology: 6, 41-53, 1992. PubMed PMID No1320667

9.Detection of Herpes Simplex Virus type 2 Antigens in biopsies from Carcinoma of the Uterine Cervix by immunofluorescence Technique: V. Thankamani, T.V. Kumari and D.M.Vasudevan: J. Experimental Pathology, 2, 123-133, 1985. PubMed PMID No 2431113

10.Detection of HSV-1 DNA segments in human oral cancer biopsies by dot-blot and in situ hybridisation Technique: D.M.Vasudevan, K.R. Shanavas, T. Vijayakumar and A. Antony: J. Experimental and Clinical Cancer Research, 10, 291-294, 1991.

11.Detection of Herpes Simplex Virus Type-2 DNA and Human Papilloma Virus DNA sequences in cervical carcinoma tissue by Molecular Hybridisation: V. Thankamani, T.V. Kumari and D.M.Vasudevan: J. Experimental Pathology, 6, 55-64, 1992. 1320668

12.Anti HHV-6 Antibodies in Normal Population and in Cancer Patients in India: K.R. Shanavas, V. Kala, D.M.Vasudevan, T. Vijayakumar and M. Yadav: J. Experimental Pathology, 6, 95-105, 1992. Pubmed PMID No1320669

13.Frequent detection of Human Herpes virus 6 in oral carcinoma: M.Yadav, A. Chandrasekharan, D.M.Vasudevan and D.V. Aablashi: J. National Cancer Institute., 86, 1792-1794, 1994. PubMed PMID No7966419

14. Viruses in Human Oral Cancers: Invited Review Article: D.M.Vasudevan and T.Vijayakumar: J. Experimental and Clinical Cancer Research, 17, 27-31, 1998. PubMed PMID No9646230

15.Quantitation of Fc receptor-bearing T Lymphocytes (TG and TM) in Oral Cancer: B.Prabha and D.M.Vasudevan: Cancer (USA), 52, 1837-1840, 1983. PubMed PMID No.6605187

16.High Affinity Rosette Forming Cells in Carcinoma of Oral Cavity, Uterine Cervix and Breast: T. Vijayakumar and D.M.Vasudevan: Cancer Letters: 27, 339-345, 1985. PubMed PMID No 4016728

17.Leucocyte Adherence Inhibition Assay (LAI) in Cancer of the Oral Cavity: B.Prabha, T.V.Kumari and D.M.Vasudevan: European. J. Cancer and Clinical. Oncology. 20, 891-897, 1984.

18. Immunoglobulins in Carcinoma Cervix: D.M.Vasudevan, K. Balakrishnan and G.P.Talwar: Indian J. Medical Research, 59, 1653-1657, 1971. PubMed No PMID 5159002.

19.Detection and Quantitation of Immunoglobulins associated with Tumours of Human Oral Cavity: S.Pillai, V.K.Sasidharan, B.Prabha, S. Vivekanandan and D.M.Vasudevan: Indian. J. Cancer, 19, 44-46, 1982. PMID 7106832

20.Quantitation of Tissue Immunoglobulins in Premalignant and Malignant Lesions of the Oral Cavity: A. Ravindran, P. Remani, D.M.Vasudevan and T. Vijayakumar: Cancer Journal, 1, 135-136, 1986.

21.Demonstration of Circulating Immune Complexes in Oral and Cervical Cancer Patients: P.N. Raghunath, P.C. Sudheeran, C.D. Joseph and D.M.Vasudevan: J. of Experimental and Clinical Cancer Research: 6, 173-178, 1987.

22.Serum Immunoglobulins in patients with Carcinoma of Oral Cavity, Uterine Cervix and Breast: T. Vijayakumar, Ravindran Ankathil, P. Remani, V.K. Sasidharan, K.K. Vijayan and D.M.Vasudevan: Cancer Immunology and Immunotherapy, 22, 76-79, 1986. PubMed PMID No3708632

23.Evaluation of Three Antigen Nonspecific methods for detecting Circulating Immune Complexes in Oral Cancer: P.N. Raghunath, C.D. Joseph, P.C. Sudheeran and D.M.Vasudevan: Indian J. Clinical. Biochemistry, 2, 85-89, 1987.

24.Circulating Immune Complex as a Biological Marker for Solid Tumours: T. Vijayakumar, P. Remani, R. Ankathil, V.K. Sasidharan, K.K. Vijayan and D.M.Vasudevan: J. Experimental and Clinical Cancer Research: 5, 257-261, 1986.

25.Demonstration of Tumour Associated Antigen in Human Oral Cancers: M .Abdul Khader, A. Ravindran, L. Sudha, T.V. Kumari and D.M.Vasudevan: Indian J. Medical Research: 74, 428-432, 1981. PubMed No. PMID 7319582

26.Demonstration of an Oral Cancer Associated Antigen: K.R. Shanavas, V.N. Bhattathiri, T. Vijayakumar and D.M.Vasudevan: Oral Oncology, Vol.II, Ed: A.K. Varma, Macmillan India Ltd., Delhi, p.117-120, 1991.

27. Effect of Neuraminidase on electrophoretic mobility and immune cytolysis of human uterine cervix carcinoma cells: D.M.Vasudevan, K.Balakrishnan and G.P.Talwar: International J. Cancer, 6, 506-516, 1970. PubMed, PMID No. 5486608

28.Glycosaminoglycan composition of Human Oral Cancer Tissue: P. Sreekumar, V.K. Sasidharan, M.A. Khadar and D.M.Vasudevan: Indian Journal of Cancer, 18, 258-261, 1981. Pubmed, PMID No. 7341425

29.Tissue Binding Patterns of Lectins in Premalignant and Malignant lesions of the Oral Cavity: K.K. Vijayan, P. Remani, V.M. Haseena Beevi, Ravindran Ankathil, T. Vijayakumar, R. Rajendran, Joy Augustine and D.M.Vasudevan: J. of Experimental Pathology, 3, 295-304, 1987. PubMed PMID No3449613

30.Canavalia virosa lectin and its tissue binding pattern in squamous cell carcinoma of oral cavity: K.K. Jayavardhanan, K. Suresh, K.R. Panikkar, P.B. Ajayakumar and D.M.Vasudevan: J. Experimental and Clinical Cancer Research: 14, 215-219, 1995.

31.Jack Fruit Lectin Binding Pattern in Benign and Malignant Lesions of Breast: P. Remani, Joy Augustine, K.K. Vijayan, R. Ankathil, D.M.Vasudevan, M. Krishnan Nair and T. Vijayakumar: In vivo: 3, 275-278, 1989. PubMed PMID No2519866;

32.Jack Fruit Lectin Binding Pattern in Carcinoma of the Uterine Cervix: P. Remani, A.Joy, K.K.Vijayan, A.Ravindran, V.M. Haseena Beevi, D.M.Vasudevan and T. Vijayakumar: J. of Experimental Pathology, 5, 89-96, 1990. PubMed PMID No2094774

33.Modulatory potency of drum stick lectin on the host defense system: K.K. Jayavardhanan, K. Suresh, K.R. Panikkar and D.M.Vasudevan: Journal of Experimental and Clinical Cancer Research. 13, 205-209, 1994.

34.Augmentation of Murine NK cell and ADCC activities by Phyllanthus Emblica, a New Immunomodulator: K. Suresh and D.M.Vasudevan: Journal of Ethnopharmacology. 44, 55-60, 1994. PubMed PMID No7990505

35. Assessment of Immunological parameters during Tumour Development in a Murine Model: K. Suresh, K. Nirmala, K. Kuruvilla and D.M.Vasudevan: Indian J. Experimental Biology., 27, 497-501, 1989 June. PubMed PMID No2583740.

36.Kinetics of NK cell Activity during Tumour Development in a Mouse model: K. Suresh and D.M.Vasudevan: The Cancer Journal: 4, 97-102, 1991.

37.Adoptive Immunotherapy using Human Recombinant Interleukin-2 Activated Specific Cytotoxic T Lymphocytes: K. Suresh, K. Nirmala and D.M. Vasudevan: J. Experimental and Clinical Cancer Research, 9, 155-160, 1990.

38.Natural Killer Cell and Antibody Dependent Cellular Cytotoxicity in gastric carcinoma patients: Modulatory effect of IL-2 and 5-Fluoro Uracil :K.Suresh, K.K. Jayavardhanan, K. Shyamasundar, C.S. Kuttappan and D.M.Vasudevan: Oncology Reports: 1, 439-443, 1994.

39.Defective Interleukin-2-R gene expression in gastric carcinoma patients: K. Suresh, K.K. Jayavardhanan and D.M.Vasudevan: Immunological Investigations (USA). 24, 565-571,1995. PubMed PMID No7622194

40.Enhancement of Natural Cell Mediated Cytotoxicity in Gastric Carcinoma patients followed by 5-Fluoro Uracil Treatment: K. Suresh, K.K. Jayavardhanan and D.M.Vasudevan: Biochemical Archives: 11, 27-34, 1995.

41. Increased cytolytic effect of Immune Lymphocytes in a Syngeneic tumour system following simple purification procedures: D.M.Vasudevan, K.T. Brunner and J-C. Cerottini: British J.Cancer: 28, 35-36, 1973 August. PubMed, PMID No.4804289

42. Detection of Cytotoxic T Lymphocytes in the EL-4 Leukemia system; Increased activity of Immune Spleen and Peritoneal cells following pre-incubation and fractionation procedures: D.M. Vasudevan, K.T. Brunner and J-C. Cerottini: International J. Cancer, 14, 301-313, 1974. PubMed, PMID 4549212.

43. Detection of Cytotoxic T Lymphocytes in Tumour Bearing Animals; procedures to isolate highly active populations; D.M.Vasudevan and T.Vijayakumar: Indian J Cancer, 14, 354-360, 1977. PubMed No. PMID 306380

44. Lymphocyte mediated Cytotoxicity, Mechanisms and Relationship to immunity; K.T. Brunner, F. Plata, D.M.Vasudevan and J-C. Cerottini: In Host Defense against Cancer and its Potentiation; Ed. D.Mizuno et al, University of Tokyo, p. 43-53, 1975.

45.Role of Levamisole Immunotherapy as an Adjuvant to Radiotherapy in Oral Cancer-I. A three year clinical follow up: T.K. Padmanabhan, B. Prabha and D.M.Vasudevan: Neoplasma: 34, 627-632, 1987. PubMed PMID No 3696303

46.Role of Levamisole Immunotherapy as an Adjuvant to Radiotherapy in Oral Cancer-II. Lymphocytes Sub-populations: B. Prabha, T.K. Padmanabhan and D.M.Vasudevan: Neoplasma, 35, 235-242, 1988. PubMed PMID No3259681

47.Role of Levamisole Immunotherapy as an Adjuvant to Radiotherapy in Oral Cancer-III. Immune responses: B. Prabha, P. Remani, T.K. Padmanabhan and D.M.Vasudevan: Neoplasma, 35, 617-625, 1988. PubMed PMID No3221935

48.Presence of a Receptor for the Active Components of Iscador in Ascites fluid of Tumor bearing Mice: G. Kuttan, D.M.Vasudevan and R. Kuttan: Cancer letters, 48, 223-227, 1989. PubMed PMID No2605571

49.Effect of a Preparation from Viscum Album on Tumour Development in vitro and in Mice: G. Kuttan, D.M.Vasudevan and R. Kuttan: J.Ethnopharmacology, 29, 35-41, 1990. PubMed PMID No2345458

50.Tumour Reducing Activity of an Isolated Active Ingredient from Mistletoe extract and its possible mechanism of action: G. Kuttan, D.M.Vasudevan and R. Kuttan: J. Experimental and Clinical Cancer Research, 11, 7-12, 1992.

51.Isolation and Identification of a Tumour Reducing Component from Mistletoe extract (Iscador): G. Kuttan, D.M.Vasudevan and R. Kuttan: Cancer letters, 41, 307-314, 1988. PubMed PMID No3409209

52.Radiation induced Peripheral Blood Changes in Postnatally Developing Mice and its Chemical Modification by MPG: S. Kumar, C.D. Joseph, D.M.Vasudevan and P.Umadevi: J. Radiobiol. Radiother. 25, 591-596, 1984. PMID 6505179.

53.Studies with MPG against the side effects of Cyclophosphamide (Endoxan); a preliminary study: S. Kumar, P.N. Raghunath, C.D. Joseph and D.M.Vasudevan: Indian Journal of Cancer, 22, 228-232, 1985. PubMed PMID No 3843325

54.Role of 2’-mercaptopropionylglycine (MPG) against the toxicity of Cyclophosphamide in Normal and Tumour Bearing Mice: P. Bhanumathy, S. Kumar and D.M.Vasudevan: Indian J. Experimental Biology, 24, 767-770, 1986. PubMed PMID No 3583333

55.Radioprotective effect of MPG on the Peripheral Blood of Mammals; a review: S. Kumar, D.M.Vasudevan, C.D. Joseph and P. Uma Devi: Indian. J. Hematology. 1, 163-167, 1983. 56.Creatine Phosphokinase and Cardiotoxicity in Adriamycin chemotherapy and its modification by WR-1065: P. Bhanumathi, E.B. Saleesh and D.M.Vasudevan: Biochemical Archives, 8, 335-339, 1992.

57.Modification of Adriamycin / cyclophosphamide induced immune suppression by Aminothiol: P. Bhanumathi, E.B. Saleesh and D.M.Vasudevan: Biochemical Archives, 10, 111-116, 1994.

58.Immunological Phenomena in Human Oral Carcinoma in India (Invited Review article): T. Vijayakumar, K.R. Shanavas and D.M.Vasudevan: Oral Oncology, European J. Cancer, 29, 181-185, 1993. PubMed PMID No8298421

59 Biochemical parameters in Alcoholic Liver Injury: Subir Kumar Das, Prasunpriya Nayak and D.M. Vasudevan, Trends Clin. Biochem. Lab. Medicine, 227-233, 2003

60. Should we use carbohydrate deficient transferrin as a marker for alcohol abusers? Subir Kumar Das and D.M. Vasudevan: Ind J Clin Biochemistry, 19, 36-44, 2004.

61. Effects of long-term ethanol consumption on adhesion molecules in liver: Das SK, Mukherjee S, and Vasudevan D.M: Indian J. Exp. Biol., 48, 394-401, 2010. (PMID No: 20726338)

62. Essential factors associated with hepatic angiogenesis. Das SK, Vasudevan DM: Life Sciences 81 (23-24): 1555-1564. 2007. PubMed PMID No17950364

63. Genesis of Hepatic Fibrosis and its biochemical markers: S. Kumar Das and D.M. Vasudevan: Scand. J. Clin. Lab Invest. 68, 260-269, 2008. PubMed PMID No18609066;

64.Effect of long term ethanol consumption on cell death in liver: Subir K Das, Sukhes Mukherjee, Vasudevan DM: Ind J of Clinical Biochemistry: 26, 84-87, 2011

65.Consequences of Ethanol Consumption: SK Das, P Nayak and D.M. Vasudevan: Journal of Indian Society of Toxicology.: 1, 1, p.1-10, 2005.

66.Alcohol: Its health and social impact in India: Subir Kumar Das, V. Balakrishnan and D.M.Vasudevan: National Medical Journal of India, 19, 94-99, 2006. PubMed PMID No 16756199

67.Biochemical Diagnosis of Alcoholism: Subir Kumar Das, and D.M.Vasudevan : Ind. J. Clinical Biochemistry: 20, 35-42, 2005.

68. Biomarkers of alcoholism; an updated review: Das SK, Dhanya L, Vasudevan DM: Scand. J. Clin. Lab. Invest. 68, 81-92, 2008. PubMed PMID No17852805.

69.Monitoring oxidative stress in patients with non-alcoholic and alcoholic liver diseases: Subir Kumar Das and D.M. Vasudevan: Indian J of Clinical Biochemistry, 20, 24-28, 2005.

70: Effect of ethanol on liver antioxidant defense systems: a dose dependent study: Subir Kumar Das and D.M.Vasudevan. Indian J Clinical Biochemistry 20, 80-84, 2005.

71. Time-dependent effects of ethanol on blood oxidative stress parameters and cytokines: Subir Kumar Das, Sowmya Varadhan, Geetanjali Gupta, Sukhes Mukherjee, Dhanya L, D N Rao and D M Vasudevan: Ind. J. Biochem. Biophysics, 46, 116-121, 2009.

72.Protective effects of silymarin, a milk thistle (Silybium marianum) derivative on ethanol-induced oxidative stress in liver: S.K. Das and D.M. Vasudevan: Indian Journal of Biochemistry & Biophysics, 43, 306-311, October 2006. PubMed PMID No17133738.

73.Modulation of lecithin activity by vitamin-B complex to treat long term consumption of ethanol induced oxidative stress in liver: Subir Kumar Das and D.M. Vasudevan, Indian Journal of Experimental Biology, 44, 791-801, 2006. PubMed PMID No17131909

74. Effect of lecithin with vitamin B complex and tocopheryl acetate on long term effect of ethanol induced immunomodulatory activities: Subir Kumar Das, Geetanjali Gupta, D.N. Rao and D.M. Vasudevan: Indian Journal of Experimental Biology, 45, 683-688, 2007. PubMed PMID No17877144

75. Medicinal properties of milk thistle with special reference to silymarin– An overview: Subir Kumar Das, Sukhes Mukherjee and D M Vasudevan: Natural Product Radiance, Vol.7, 182-192, 2008.

76. Alcohol Induced Oxidative stress, Mini Review: Subir Kumar Das and D.M. Vasudevan, Life Sciences, 81, 177-187, 2007. PubMed PMID No17570440.

77. Time Dependent Effects of Ethanol in Blood Oxidative Stress Parameters and Cytokines: Subir Kumar Das, Sowmya Varadhan, Geetanjali Gupta, Sukhes Mukherjee, Dhanya L, D N Rao and D M Vasudevan: Indian Journal of Biochemistry and Biophysics, 46, 116-121, 2009. PubMed PMID No19374264

78.Oxidative Stress is the Primary Event: Effects of Ethanol Consumption in Brain": Subir Kumar Das, Hiran KR, Sukhesh Mukherjee and D.M. Vasudevan: Ind. J. Clinical Biochemistry, 22, 99-104, 2007.

79. Consequences of Alcohol Consumption on Neurotransmitters – An Overview: Sukhes Mukherjee, Subir Kumar Das, Kannan Vaidyanathan and D.M. Vasudevan: Current Neurovascular Research, 5, 266-272, 2008. PubMed PMID No19133404.

80. Non-alcoholic fatty liver disease: An under-recognized cause with emerging importance: Subir Kumar Das, Sukhes Mukherjee and D.M.Vasudevan: Current Science 90, 659-665, 2006.

81.Role of Plasma Amino acids and GABA in Alcoholic and Non-alcoholic Fatty Liver Disease; a pilot study: S. Mukherjee, K. Vaidyanathan, D.M.Vasudevan and S.K.Das: Indian Journal of Clinical Biochemistry, 25, 37-42, 2010.

82.Clinicopathological spectrum of non-alcoholic fatty liver disease among patients in Kerala: Subir Kumar Das; Sukhes Mukherjee, Gaurav Pandey, V Balakrishnan, and D M Vasudevan: Indian Journal of Biochemistry, 24, 155-158, 2009.

83.Evaluation of blood oxidative stress related parameters in alcoholic liver disease and non-alcoholic fatty liver disease: S. Kumar Das, V. Balakrishnan, S. Mukherjee, and D.M. Vasudevan, Scand. J. Clin Lab invest, 68, 323-334, 2008. PubMed PMID No18609067.

84. Role of cytokines and insulin resistance in non-alcoholic fatty liver disease. Das SK, Mukherjee S, Pandey G, Varadhan S, Dhanya L, Balakrishnan V, Vasudevan DM : Clin Chem. 54: 111-112. 2008.

85. Dietary Grape (Vitis vinifera) feeding attenuates ethanol-induced oxidative stress in blood and modulates immune functions in mice: Mukherjee S, Das SK and Vasudevan DM: Indian J Biochem. Biophys: 49, 379-385, 2012.

86.Protective effect of Resveratrol and Vitamin E against Ethanol-induced Oxidative Damage in Mice; Biochemical and Immunological basis: Subir Kumar Das, Sukhes Mukherjee, Geetanjali Gupta, D. N. Rao, and D. M. Vasudevan: Indian Journal of Biochemistry and Biophysics, 47, 32-37, 2010. PubMed PMID No21086752.

87. Protective role of extracts of grape skin and grape flesh on ethanol induced oxidative stress, inflammation and histological alterations in Rat Brain". Mukherjee, Sukhes; Das, Subir; DM Vasudevan, Archives of Physiology and Biochemistry. Online link: http://dx.doi.org/10.3109/13813455.2015.1075046. Page 1-7, September 2015.

88. Effects of Ethanol Consumption on Different Organs, a Brief Overview: Sukhesh Mukherjee, Subir Kumar Das and D.M.Vasudevan: Asian Journal of Biochemistry; 2, 386-394, 2007.

89. Role of Polyphenols in diet and Nutrition; An updated Review: Sukhes Mukherjee, Subir Kumar Das and D.M. Vasudevan: Current Nutrition and Food Science. 5, 149-159, 2009.

90.Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzymes in rats. M Maneesh, H Jayalekshmi, Sanjiba Dutta, Amit Chakrabarti, and D M Vasudevan: Indian Journal of Experimental Biology 43, 445-449, 2005. PubMed PMID No15900910.

91.Role of oxidative stress in ethanol induced germ cell apoptosis – an experimental study in rats : M Maneesh, H Jayalekshmi, Sanjiba Dutta, Amit Chakrabarti and D M Vasudevan: Indian Journal of Clinical Biochemistry, 20, 62-67, 2005.

92. Effect of exogenous lecithin on ethanol induced testicular injuries in wistar rats: M Maneesh, H Jayalekshmi, Sanjiba Dutta, Amit Chakrabarti, D M Vasudevan: Ind.J. Physiology and Pharmacology, 49, 297-304, 2005. PubMed PMID No16440847.

93. Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats: M.Maneesh, Suanjiba Dutta, Amit Chakrabarti and D.M. Vasudevan: Indian Journal of Clinical Biochemistry: 22, 138-142, 2007.

94. Effect of alpha-tocopherol supplementation on renal oxidative stress and adenosine triphosphatase in ethanol treated Wistar rats : M Maneesh, H Jayalekshmi Amit Chakrabarti, Neha Alang, D M Vasudevan: Indian Journal of Experimental Biology, 47:608-610, 2009. PubMed PMID No19761047

95.Experimental therapeutic intervention with ascorbic acid in ethanol induced testicular injuries in rats: M. Maneesh, H.Jayalakshmi, Sanjiba Dutta, Amit Chakrabarti and D.M. Vasudevan: Indian Journal of Experimental Biology, 43, 172-176, 2005. PubMed PMID No15782819.

96. Effect of exogenous L-ornithine L-aspartate on ethanol-induced testicular injury in wistar rats: Maneesh Mailankot, H. Jayalekshmi, Amit Chakrabarti and D.M. Vasudevan: Indian Journal of Clinical Biochemistry, 24, 94-97, 2009.

97.Alcohol abuse-duration dependent decrease in plasma testosterone and antioxidants in males : M Maneesh, Sanjiba Dutta, Amit Chakrabarti and D M Vasudevan : Indian Journal of Physiology and Pharmacology; 50, 291-296, 2006. PubMed PMID No17193902.

98.Activity of Ocimum Sanctum (the traditional Indian Medicinal Plant) against the Enteric Pathogens: Geetha M Ballal, D.M. Vasudevan, R. Kedlaya, S. Deepa, M. Ballal: Indian Journal of Medical Sciences, 55, 434-438, 2001. PubMed PMID No12026506.

99. Inhibition of lipid peroxidation by botanical extracts of Ocimum sanctum: in vivo and in vitro studies: Geetha, R. Kedlaya and D.M. Vasudevan: Life Sciences, 76, 21-28, 2004. PubMed PMID No15532130

100. Radioprotective effects of Ocimum flavonoids on leukocyte oxidants and antioxidants in oral cancer: Reshma K, A.V. Rao, M. Dinesh and D.M. Vasudevan: Ind. J. Clin. Biochem: 23, 171-175, 2008

101. Hypolipidemic efficacy of Ocimum sanctum in the prevention of Atherogenesis in male albino rabbits: Geetha Samak, Muddanna S Rao, Rajendra Kedlaya and D.M. Vasudevan. Pharmacologyonline, 2, 115-127, 2007.

102.Tulsi: The Indian Holy power Plant: Subir Kumar Das and D.M.Vasudevan: Natural Product Radiance: 5, 279-283, 2006.

103. Effect of High fat diet without cholesterol supplementation on oxidative stress and lipid peroxidation in New Zealand White rabbits: Sabitha P, DM Vasudevan and Prakash Kamath: Journal of Atherosclerosis and Thrombosis, 17, 213-218, 2010. PubMed PMID No20032571.

104. Comparison of lipid profile and antioxidant enzymes among South Indian men consuming coconut oil and sunflower oil: P Sabitha, Kannan Vaidyanathan, D M Vasudevan and Prakash Kamath: Ind. J of Clinical Biochemistry, 24, 76-81, 2009.

105. Assessment of small dense LDL particles among subjects consuming ccoconut oil or sunflower oil as cooking medium by using LDL-cholesterol / LDL-apoB ratio as a surrogate marker : Sabitha Palazhy, Prakash Kamath, DM Vasudevan :: J of Medical Nutrition and Nutraceuticals : 3, 39-44, 2014.

106. Composition of plasma and atheromatous plaque among coronary artery disease subjects consuming coconut oil or sunflower oil as the cooking medium. Palazhy S, Kamath P, Rajesh PC, Vaidyanathan K, Nair SK, Vasudevan DM.: Journal American College of Nutrition.; 31: 392-396, 2012. PMID No. 23756582.

107. A randomized study of coconut oil versus sunflower oil on cardiovascular risk factors in patients with stable coronary artery disease : M.Vijayakumar, DM Vasudevan, KR Sundaram, Sajitha Krishnan, Sandya Nandakumar, Rajiv Chandrasekharan, Navin Mathew; Indian Heart Journal, 68, 498-506, 2016.

108. Elevated oxidative stress among coronary artery disease patients on statin therapy: a cross sectional study: Sabitha, Palazhi, Prakash Kamath, DM Vasudevan; Indian Heart Journal, 67; 227-232, 2015.

109: Health benefits of coconut oil: DM Vasudevan: Cocoinfo International, 18, 6-8, 2011.

110. Coconut oil and health controversy, a review: DM Vasudevan: International Journal of Health and Rehabilitation Sciences, 2, 109-116, 2013.

111. Diagnosis of Major Organic Acidurias in Children; two years’ experience at a tertiary care centre: Narayanan MP, Vaidyanathan Kannan, Vinayan KP, Vasudevan DM: Ind J Clinical Biochemistry, 26, 347-353, 2011.

112. Analysis of gene mutations among south Indian patients with maple syrup urine disease; identification of four novel mutations: MP Narayanan, Krishna Kumar N Menon and DM Vasudevan: Ind J Biochem and Biophysics, 50, 442-446, 2013.

113.Organic acidurias; an updated review: Kannan Vaidyanathan, MP Narayanan, DM Vasudevan: Ind J Clin Biochem: 26, 319-325, 2011.

114. Inborn Errors of Metabolism and Brain Involvement, 5 years’ Experience from a Tertiary Care centre in South India: Kannan Vaidyananthan, MP Narayanan and DM Vasudevan : in Brain Damage, Bridging Between Basic Research and Clinics; Ed Alina Gonzalez-Quevedo; InTech; 57-78; 2012


External links